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| Registros recuperados: 269 | |
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| Ili,Carmen G; Brebi,Priscilla; Garcia,Patricia; Leal,Pamela; Lopez,Jaime; Tapia,Oscar; Letelier,Pablo; Weber,Helga; Castillo,Jonathan; Roa,Juan C. |
| Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected... |
| Tipo: Journal article |
Palavras-chave: Apoptosis; Proliferation; Cervical cancer; SiRNA; C-FLIPL. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036 |
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| Guo,Jian-Ru; Chen,Qian-Qian; Lam,Christopher Wai-Kei; Zhang,Wei. |
| BACKGROUND: We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). RESULTS: MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin... |
| Tipo: Journal article |
Palavras-chave: Karanjin; Cell cycle; Apoptosis; Cancer therapy. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100040 |
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| Tang,J.; Jiang,Y.; Tang,Y.; Chen,B.; Sun,X.; Su,L.; Liu,Z.. |
| Gut-derived endotoxin and pathogenic bacteria have been proposed as important causative factors of morbidity and death during heat stroke. However, it is still unclear what kind of damage is induced by heat stress. In this study, the rat intestinal epithelial cell line (IEC-6) was treated with heat stress or a combination of heat stress and lipopolysaccharide (LPS). In addition, propofol, which plays an important role in anti-inflammation and organ protection, was applied to study its effects on cellular viability and apoptosis. Heat stress, LPS, or heat stress combined with LPS stimulation can all cause intestinal epithelial cell damage, including early apoptosis and subsequent necrosis. However, propofol can alleviate injuries caused by heat stress, LPS,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Propofol; Heat stress; Lipopolysaccharides; Apoptosis; Intestinal epithelial cells. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600507 |
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| Sun,PengPeng; Luo,You; Wu,Xin Tong; Ansari,Abdur Rahman; Wang,Jing; Yang,KeLi; Xiao,Ke; Peng,KeMei. |
| Boron is an essential trace element which plays an important role in process of metabolism and the function of the tissues. However, the effects of boron on the intestinal cells in African ostrich chicks are poorly reported. Therefore, this study was designed to investigate the role of boron on proliferation and apoptosis of the intestinal cells. A total of 36, ten day-old ostrich chicks were randomly divided into six groups and fed on the same basal diet supplemented with 0, 40, 80, 160, 320 and 640 mg/L boric acid in drinking water for 80 days. Proliferatingcell nuclearantigen (PCNA) wasused to test the proliferation indexof intestine in different group byimmunohistochemicalstaining (IHC). Apoptoticcellsofintestinewere detectedbyDutp-biotin nick end... |
| Tipo: Journal article |
Palavras-chave: Apoptosis; Boron; Intestine; Ostrich chicks; Proliferation. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300002 |
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| Soto-Domínguez,Adolfo; Serrano-Gallardo,Luis B; García-Garza,Rubén; Ruíz-Flores,Pablo; Morán-Martínez,Javier; Nava-Hernández,Martha P; Martínez-Pérez,Edith F. |
| Ruta graveolens es una planta nativa del Mediterráneo Oriental y del área Sur Occidental de Asia, de esta planta se han aislado más de 120 compuestos químicos. En un estudio previo en nuestro laboratorio se observó que un extracto acuoso de R. graveolens causó necrosis y alteraciones morfológicas sugestivas de apoptosis sobre el hígado de rata Wistar. El objetivo del presente estudio, fue evaluar la inducción de apoptosis y el posible efecto antiproliferativo in vivo de un extracto acuso de R. graveolens del norte de México, mediante métodos inmunohistoquímicos. Se utilizaron 25 ratas Wistar y se dividieron en 5 grupos (n=5). El grupo 1 correspondió al grupo control negativo, el grupo 2 o control positivo se trató con 100 mg de dexametasona/kg/día. Los... |
| Tipo: Journal article |
Palavras-chave: Ruta graveolens; Apoptosis; Efecto antiproliferativo. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400031 |
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| Zhang,Y; Yang,H; Wu,X; Deng,M; Li,Z; Xu,Z. |
| ABSTRACT Avian leukosis virus subgroup J (ALV-J), a member of the retroviridae family, can infect both broilers and layers and induce a spectrum of different neoplasms, resulting in serious economic losses in poultry production. Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, has demonstrated remarkable anti-inflammatory and cancer chemopreventive effects in many animal tumor bioassays, cell culture systems and epidemiological studies. To assess the antiviral effects of EGCG on ALV-J-induced cell apoptosis in vitro, DF-1 cells were treated with different EGCG concentrations (0, 5, 10, 20 and 40 µg/mL), and their antiviral effects were examined at different time points (0, 24, 48, 72 and 96 h) using a variety of assays. EGCG... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ALV-J; EGCG; NF-kB; DF-1 cell; Apoptosis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100323 |
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| Wang,J.; Lu,M.L.; Dai,H.L.; Zhang,S.P.; Wang,H.X.; Wei,N.. |
| This study investigated the in vitro and in vivo antiproliferative activity of esculetin against hepatocellular carcinoma, and clarified its potential molecular mechanisms. Cell viability was determined by the MTT (tetrazolium) colorimetric assay. In vivo antitumor activity of esculetin was evaluated in a hepatocellular carcinoma mouse model. Seventy-five C57BL/6J mice were implanted with Hepa1-6 cells and randomized into five groups (n=15 each) given daily intraperitoneal injections of vehicle (physiological saline), esculetin (200, 400, or 700 mg·kg-1·day-1), or 5-Fu (200 mg·kg-1·day-1) for 15 days. Esculetin significantly decreased tumor growth in mice bearing Hepa1-6 cells. Tumor weight was decreased by 20.33, 40.37, and 55.42% with increasing doses of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Esculetin; Apoptosis; Hepatocellular carcinoma; Mitochondrial-dependent pathway; Antitumor activity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300245 |
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| Lv,Dan; Pan,Li-hong; Zhang,Ren; Yang,Jie; Chen,Hao; Wen,Yanzhang; Huang,Mi; Ma,Xinhua; Wang,Qiang; Yang,Xinzhou. |
| Hepatocellular carcinoma is one of the most prevalent malignancies and a leading cause of cancer-related mortality worldwide. However, the therapies to prevent hepatocellular carcinoma are still limited and the emergence of drug resistance leads to the development of new anti-cancer drugs and combinational chemotherapy regimens. Our study was aimed to explore the anticancer effects of the essential oil extract (EEEO) from Euphorbia esula which has been widely used in traditional Chinese folk medicine and possessed potential cytotoxic effects in several human tumor cells. However, the mechanisms of EEEO-induced anti-proliferation and apoptosis have not been completely elucidated. In this study, EEEO was prepared by hydro-distillation and the main chemical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Euphorbia esula; Hepatocellular carcinoma; Apoptosis; Reactive oxygen species; Mitochondrial pathway. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100506 |
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| Cavaliere,Victoria; Glickman,Ariel; Solari,Liliana; Gammella,Daniel; Hajos,Silvia; Álvarez,Élida. |
| La apoptosis es un proceso fisiológico crítico para el crecimiento celular, regulado por factores pro y anti-apoptóticos. Defectos en su control llevan a una ventaja selectiva en la sobrevida celular favoreciendo el desarrollo de patologías neoplásicas. NFkB puede promover la proliferación celular por lo que el uso de inhibidores selectivos lleva a un aumento de la apoptosis. El objetivo del presente trabajo fue caracterizar la línea celular PL104, obtenida de células de punción de médula ósea de una paciente con diagnóstico de leucemia mieloide aguda (LMA) atípica y evaluar la susceptibilidad de la misma frente a los quimioterápicos Doxorubicina (DOX), Vincristina (VCR), Gemcitabina e inhibidores de NFkB, CAPE y MG-132. El fenotipo evaluado por citometría... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leucemia mieloide aguda; Apoptosis; Factor de trascripción nuclear kB; Doxorubicina; Vincristina; Gemcitabina; Ácido cafein fenil etil éster; MG-132. |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572006000100004 |
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| Gervais, Ophelie. |
| The flat oyster, Ostrea edulis, is the European endemic oyster species. Its production has been reduced because of overfishing and diseases including bonamiosis. Massive mortalities observed on the Pacific cupped oyster these last years explained the wish of shellfish farmers to diversify their production and their revival of interest for this patrimonial oyster species. However, bonamiosis due to the protozoan, Bonamia ostreae, is still a major problem for the production of this species. Measures to control the disease are limited and require a better knowledge of the interactions between the flat oyster and the parasite. Previous studies have suggested the involvement of apoptosis in flat oyster defense mechanisms against B. ostreae. This mechanism is... |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Bonamia ostreae; Apoptose; Flat oyster; Ostrea edulis; Bonamia ostreae; Apoptosis. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00370/48142/48243.pdf |
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| Palma,Priscila F.R.; Baggio,Giovana L.; Spada,Celso; Silva,Renata da; Ferreira,Silvia Inês A.C.P.; Treitinger,Aricio. |
| Evaluation of apoptosis by flow cytometry is generally accomplished by methods that use annexin V-FITC as vital dye, which access phosphatidylserine exposed on the external membrane at the beginning of this process. In addition, the concomitant use of propidium iodide makes possible to verify the characteristic nuclear alterations in the late stages of apoptosis, as a consequence of the increase in membrane permeability. On the other hand, the use of calcein-AM in association with ethidium homodimer (EthD-1) allows the evaluation of cell apoptosis through detection of esterase activity and cellular membrane physical and chemical alterations. The aim of this study was to compare the sensibility of calcein-AM and EthD-1 with annexin V-FITC and propidium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Flow cytometry; Annexin V; Propidium iodide; Calcein-AM; Ethidium homodimer. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000200003 |
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| Linardi,C.C.G.; Martinez,G.; Velloso,E.D.R.P.; Leal,A.M.; Kumeda,C.A.; Buccheri,V.; Azevedo,R.S.; Peliçario,L.M.; Dorlhiac-Llacer,P.. |
| Eighty-six newly diagnosed multiple myeloma (MM) patients from a public hospital of São Paulo (Brazil) were evaluated by cIg-FISH for the presence of del(13)(q14), t(4;14)(p16.3;q32) and del(17)(p13). These abnormalities were observed in 46.5, 9.3, and 7.0% of the patients, respectively. In order to identify the possible role of del(13)(q14) in the physiopathology of MM, we investigated the association between this abnormality and the proliferative and apoptotic indexes of plasma cells. When cases demonstrating t(4;14)(p16.3;q32) and del(17)(p13) were excluded from the analysis, we observed a trend towards a positive correlation between the proportion of cells carrying del(13)(q14) and plasma cell proliferation, determined by Ki-67 expression (r = 0.23, P... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Multiple myeloma; Cell proliferation; Apoptosis; Prognosis; Fluorescence in situ hybridization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100012 |
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| DosReis,G.A.. |
| Infection with the protozoan parasite Trypanosoma cruzi leads to Chagas disease, which affects millions of people in Latin America. Infection with T. cruzi cannot be eliminated by the immune system. A better understanding of immune evasion mechanisms is required in order to develop more effective vaccines. During the acute phase, parasites replicate extensively and release immunomodulatory molecules that delay parasite-specific responses mediated by T cells. This immune evasion allows the parasite to spread in the host. In the chronic phase, parasite evasion relies on its replication strategy of hijacking the TGF-β signaling pathway involved in inflammation and tissue regeneration. In this article, the mechanisms of immune evasion described for T. cruzi... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma cruzi; T cells; GPI-anchors; TGF-β; Mucin; Apoptosis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200001 |
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| Alvarez,M; Urbina,G; Perdomo,L. |
| In the field of studies of acute toxicity induced by bacterial agents, Shiga toxins have been relevant due to the severity of the extra-intestinal diseases they cause. Numerous studies have shown that Shiga toxin induced apoptosis in different cell types; however, this important process has been little studied in vivo experimental models. In this study, the effects of excretion products of Shigella dysenteriae, in which Shiga toxin is present, were investigated on early larval stages of Zebrafish, an animal model with many advantages over other in vivo experimental models traditionally used. Both the collection of eggs and larvae of Zebrafish, and the product from excretion from Shigella dysenteriae (SdyEP) were performed according to laboratory standards.... |
| Tipo: Journal article |
Palavras-chave: Zebrafish (Danio rerio); Shigella dysenteriae; Apoptosis; Acridine Orange; Ethidium Bromide. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100014 |
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| Registros recuperados: 269 | |
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